Rehabilitation of degraded forests in Asia, Volumes 23-270

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Keywords: Salmonella spp. Isolation and molecular identification of Sunshine virus, a novel paramyxovirus found in Australian snakes. This paper describes the isolation and molecular identification of a novel paramyxovirus found during an investigation of an outbreak of neurorespiratory disease in a collection of Australian pythons. The sequence appears to contain all the coding regions of the genome, including the following predicted paramyxoviral open reading frames ORFs : 3'--Nucleocapsid N --putative Phosphoprotein P --Matrix M --Fusion F --putative attachment protein--Polymerase L '.

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There is also a nucleotide ORF between the N and putative P genes that may be an additional coding region. Phylogenetic analyses of the complete N, M, F and L genes support the clustering of this virus within the family Paramyxoviridae but outside both of the current subfamilies: Paramyxovirinae and Pneumovirinae. We propose to name this new virus, Sunshine virus, after the geographic origin of the first isolate --the Sunshine Coast of Queensland, Australia. Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops.

Ten strains of endophytic diazotroph were isolated and identified from the plants collected from three different agricultural crop species, wheat, rice and maize, using the nitrogen-free selective isolation conditions. The nitrogen-fixing ability of endophytic diazotroph was verified by the nifH-PCR assay that showed positive nitrogen fixation ability. These representative genus are not endophytic diazotrophs in the conventional sense.

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They may have obtained nitrogen fixation ability through lateral gene transfer, however, the evolutionary forces of lateral gene transfer are not well known. Molecular identification results from 16S rRNA analyses were also confirmed by morphological and biochemical data. The molecular identification of Streptococcus equi subsp.

To identify Streptococcus equi subsp. In addition, the hypervariable region of the seM gene of S. Of the samples, 35 tested positive for S. Thirty-two confirmed samples were from horses with a clinical diagnosis of strangles and three were from horses where clinical information was unavailable. Strain typing demonstrated that two novel seM alleles of S.

The application of PCR for the laboratory confirmation of strangles allowed for a rapid and sensitive identification of S. Moreover, seM typing revealed that within the samples examined two strains of S. PCR reduces the time required to obtain laboratory confirmation of strangles compared with culture methods. It also has greater sensitivity in detecting S. Additionally, seM molecular typing can differentiate between bacterial strains, assisting in the monitoring of local strains of S. Introduction Pityriasis Versicolor PV is a common health problem caused by genus Malassezia, a lipophilic fungi found as a part of the normal flora of skin.

Although PV is common in Egypt, there is little information regarding the Malassezia species distribution in PV patients to date. Aim To spot a light on the distribution and clinico-epidemiological features of the Malassezia species in PV patients and healthy individuals that were established by conventional phenotypic and molecular techniques. Materials and Methods A cross-sectional study including individuals; clinically suspected PV patients attending Mansoura University Hospitals, Egypt and 30 healthy control individuals, was carried out.

Characterization of Malassezia species was performed phenotypically by conventional, culture-based methods and biochemical tests. The association of Malassezia species with epidemiological profile and clinical characteristics was studied. Results A By phenotypic methods, only Most species were isolated from hypopigmented lesions of PV patients aged between years.

Neck and back were the most common affected sites. Only M. Evaluation of molecular markers for Phytophthora ramorum detection and identification using a standardized library of isolates. A number of molecular diagnostic procedures for detection of Phytophthora ramorum have been reported in the literature.

In an effort to evaluate the specificity of 10 of these techniques a standardized DNA library for isolates was assembled that included 60 described species as well as 22 taxonomically unclassified isolates. These were sent blind Rhodotorula species are emergent fungal pathogens capable of causing invasive infections, primarily fungemia.

They are particularly problematic in immunosuppressed patients when using a central venous catheter.

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In this study, we evaluated the species distribution of 51 clinical and 8 environmental Rhodotorula species isolates using the ID32C system and internal transcribed spacer ITS sequencing. Antifungal susceptibility testing and biofilm formation capability using a crystal violet staining assay were performed. Using ITS sequencing as the gold standard, the clinical isolates were identified as follows: 44 R. The environmental isolates included 7 R. Using the ID32C system, along with a nitrate assimilation test, only In the biofilm formation assay, R.

In this study, we emphasized the importance of molecular methods to correctly identify Rhodotorula species isolates and non-R. The antifungal susceptibility profile reinforces amphotericin B as the antifungal drug of choice for the treatment of Rhodotorula infections. To our knowledge, this is the first study evaluating putative differences in the ability of biofilm formation among different Rhodotorula species. Molecular identification , antifungal susceptibility profile, and biofilm formation of clinical and environmental Rhodotorula species isolates.

To our knowledge, this is the first study evaluating putative differences in the ability of biofilm formation among different Rhodotorula. Microbiological and molecular identification of bacterial species isolated from nasal and oropharyngeal mucosa of fuel workers in Riyadh, Saudi Arabia. This study aimed to determine the bacterial species colonizing the nasal and oropharyngeal mucosa of fuel workers in Central Riyadh, Saudi Arabia on a microbiological and molecular level.

Throat and nasal swab samples were obtained from 29 fuel station attendants in the period of time extending from March to May in Riyadh, Saudi Arabia.

Microbiological identification techniques were utilized to identify the bacterial species isolated. Antibiotic sensitivity was assessed for each of the bacterial isolates.


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Molecular identification techniques based on PCR analysis of specific genomic sequences was conducted and was the basis on which phylogeny representation was done for 10 randomly selected samples of the isolates. Blood was drawn and a complete blood count was conducted to note the hematological indices for each of the study participants.

Nineteen bacterial species were isolated from both the nasal cavity and the oropharynx including Streptococcus thoraltensis , alpha-hemolytic streptococci, Staphylococcus hominis , coagulase-negative staphylococci, Leuconostoc mesenteroides , Erysipelothrix rhusiopathiae and several others.

Whereas cefotaxime and azithromycin posted sensitivities of Low sensitivities isolates were compared to it.


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Most hematological indices recorded were within the normal range. In conclusion, exposure to toxic fumes and compounds within fuel products may be a contributing factor to bacterial colonization of the respiratory tract in fuel workers.

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Isolation and Molecular Identification of Streptomyces spp. Introduction: Streptomyces are a group of prokaryotes that are usually found in all types of ecosystems including water and soil. This group of bacteria is noteworthy as antibiotic producers; so the isolation and characterization of new species seemed to be crucial in introduction of markedly favorable antibiotics. Therefore, in this study we aim to isolate and characterize novel strains of Streptomyces with high antibiotic production capability. Methods: To achieve this goal, from isolates collected throughout northwest of Iran, 12 selected Streptomyces isolates which exhibited high antibacterial activity against pathogenic bacteria were subjected to PCR reaction for identification via 16S rDNA gene and random amplified polymorphic DNA RAPD pattern analysis.

Results: Analysis of morphological and biochemical characteristics and the 16S rDNA gene sequence indicated that all 12 selected isolates belonged to the genus Streptomyces. Moreover, screening of the isolates with regard to their antimicrobial activity against indicator bacteria as well as their classification using RAPD analysis revealed that GC1 and K36C5 isolates have considerable antimicrobial activity and high similarity to Streptomyces coelicolor and Sreptomyces albogriseolus, respectively.

Conclusion: Since many isolates in this study showed inhibitory effects against pathogenic bacteria, soil of northwest of Iran could be used as a rich source to be explored for novel Streptomyces strains with high potency of antibiotic production. Streptomyces are a group of prokaryotes that are usually found in all types of ecosystems including water and soil.

To achieve this goal, from isolates collected throughout northwest of Iran, 12 selected Streptomyces isolates which exhibited high antibacterial activity against pathogenic bacteria were subjected to PCR reaction for identification via 16S rDNA gene and random amplified polymorphic DNA RAPD pattern analysis. Analysis of morphological and biochemical characteristics and the 16S rDNA gene sequence indicated that all 12 selected isolates belonged to the genus Streptomyces.

Since many isolates in this study showed inhibitory effects against pathogenic bacteria, soil of northwest of Iran could be used as a rich source to be explored for novel Streptomyces strains with high potency of antibiotic production. Isolation and identification of molecular species of phosphatidylcholine and lysophosphatidylcholine from jojoba seed meal Simmondsia chinensis. A mixture of lysophosphatidylcholine LPC and phosphatidylcholine PC has been isolated by column chromatography from a jojoba meal Simmondsia chinensis extract.

Eight other molecular species of LPC and four other molecular species of PC could be assigned by comparison of the mass spectra of the isolated compounds with the spectra of the two major compounds.

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